mutS

168

DNA mismatch repair (mismatch recognition protein)

locus: BSU_17040

Molecular weight: 97.39 kDa

pI: 5.19

Protein length: 858 aa

Gene length: 2577 bp

function: DNA repair

product: mismatch recognition protein

essential: no

synonyms: mutS

List of homologs in different organisms, belongs to COG0249 (Galperin et al., 2021)

Gene

Coordinates: 1,775,745 → 1,778,321

Phenotypes of a mutant

• 60-fold increased mutation rate [Pubmed|23882084]
• a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]

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The protein

Catalyzed reaction/ biological activity

• MutS recognizes mismatches in the DNA and recruits [protein|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL] to the site of mismatch recognition
• assists DNA strand exchange by facilitating [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly, and indirectly re-engagement with the homologous 5'-end of the linear duplex [pubmed|30814990]
• modulates bidirectional [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-mediated integration of divergent sequences [pubmed|30814990]

Protein family

• DNA mismatch repair mutS family (with [protein|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU], according to UniProt)

Structure

• [PDB|1NG9] (from ''E. coli'', 39% identity) [Pubmed|12554674]
• [AF|P49849]

[wiki|Localization]

• forms foci at midcell position, the frequency of foci increases upon mismatch formation [Pubmed|21958350]
• replication-active region of the DNA, via [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN] [Pubmed|23228104]
• MutS dynamics are heterogeneous in cells, with one MutS population exploring the nucleoid rapidly, while another MutS population moves to and transiently dwells at the [wiki|replisome] region, even in the absence of appreciable mismatch formation [Pubmed|26575623]

Expression and Regulation

Operons

• genes: [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]

  description: [Pubmed|8760914]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mutL]' and '[protein|search|ymzD]' [PubMed|20525796]

Biological materials

Mutant

• GP1190 (Δ[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::''aphA3''), available in [wiki|Jörg Stülke]'s lab [Pubmed|22178973]
• 1A833 ( ''mutS''::''spec''), [Pubmed|11779496], available at [http://bgsc.org/getdetail.php?bgscid=1A833 Bacillus Genetic Stock Center]
• BKE17040 (Δ[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTAATCCCTCACTA,  downstream forward: _UP4_TTACATTAAAACGGGGTGAT
• BKK17040 (Δ[gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTAATCCCTCACTA,  downstream forward: _UP4_TTACATTAAAACGGGGTGAT

References

Reviews

• 22933559,26354434,24629484,26343983,29197672,35210609,38551349

Original Publications

• 8760914,15375129,16479537,20525796,20453097,23228104,21958350,23882084,23998896,24062730,12554674,25326311,26163658,26575623,27851738,29939310,30272207,30814990,35013597,35847079