fapR

fapR
168

repressor of fatty acid synthetic genes

locus
BSU_15880
Molecular weight
21.26 kDa
pI
5.39
Protein length
Gene length
function
regulation of fatty acid biosynthesis
product
transcriptional repressor
essential
no
synonyms
fapR, ylpC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2050 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,661,967 → 1,662,533
additional information
[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA], [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], and [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB], [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC], [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD] or downregulation of the [wiki|FapR regulon] due to specific mutations in [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [pubmed|37017514]
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The protein
Catalyzed reaction/ biological activity
FapR regulates the expression of at least 10 genes (the ''fap'' regulon). It autoregulates its own expression. Malonyl-CoA, a precursor of fatty acid biosynthesis, binds to FapR changing its conformation to a non-DNA binding state. Hence, conditions that cause malonyl-CoA accumulation, like fatty acid biosynthesis inhibition, derepress the ''fap'' regulon.
Protein family
fapR family (single member, according to UniProt)
Structure
[PDB|2F3X] (complex with effector),  [PDB|2F41] [Pubmed|16932747]
[AF|O34835]
Effectors of protein activity
malonyl-CoA and malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP]]] act as the molecular inducer of the FapR regulon [Pubmed|20201588]
Expression and Regulation
Operons
genes
[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]-[gene|8F5612395EBF90BEF4C80056EECAFC572384E7F8|plsX]-[gene|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD]-[gene|439B468A13137000FB42E9389391CB4986FFED84|fabG]-[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]
description
[Pubmed|19850612]
regulation
''[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]'': repressed in the absence of malonyl-CoA or malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP] ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [Pubmed|12737802]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
[protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]) [Pubmed|16091051], in [regulon|protein:7832489F11F606BCF637EC23BAAB41AD10237EBB|comA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16932747], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]→[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]

2024-03-15 21:31:06

Jstuelk

115

FFBE1C006C18216F03207DE6CCE7201A900066A3

A19B82B645D7251903097316A08D79733FD80DE9

Biological materials
Mutant
MGNA-B138 (ylpC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1137 NBRP B. subtilis, Japan]
BKE15880 (Δ[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCTAAACACCATCCGG,  downstream forward: _UP4_AAACATTCATAAAGGTGGAG
BKK15880 (Δ[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCTAAACACCATCCGG,  downstream forward: _UP4_AAACATTCATAAAGGTGGAG
lacZ fusion
GP3687 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
17919287,27766255,15802245,18372209
Original Publications
18384517,20201588,16932747,31113899,32671874,37017514
The [wiki|FapR regulon]
12737802

FCDE900167AE36377979E0CF7BC33C7F351B95D3

Page visits: 2610

Time of last update: 2024-03-29 01:27:25

Author of last update: Jstuelk