rpeA

168

ribulose 5-phosphate 3-epimerase

locus: BSU_15790

Molecular weight: 23.19 kDa

pI: 5.65

Protein length: 217 aa

Gene length: 654 bp

function: pentose phosphate pathway

product: ribulose 5-phosphate 3-epimerase

essential: no

ec: 5.1.3.1

synonyms: rpe, yloR

List of homologs in different organisms, belongs to COG0036 (Galperin et al., 2021)

Gene

Coordinates: 1,654,004  1,654,657

Phenotypes of a mutant

• the mutant cells are shorter than wild type cells [pubmed|34846166]

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The protein

Catalyzed reaction/ biological activity

• D-ribulose 5-phosphate --> D-xylulose 5-phosphate (according to UniProt)

Protein family

• ribulose-phosphate 3-epimerase family (single member, according to UniProt)

Structure

• [PDB|1TQJ] (from ''Synechocystis sp.'', 61% identity, 75% similarity) [Pubmed|15333955]
• [AF|O34557]

Expression and Regulation

Operons

• genes: [gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B374 (yloR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1373 NBRP B. subtilis, Japan]
• BKE15790 ([gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGATGGTGCAACCTTTATCA,  downstream forward: _UP4_TAAGCAGCTTTAGAAAAGAG
• BKK15790 ([gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGATGGTGCAACCTTTATCA,  downstream forward: _UP4_TAAGCAGCTTTAGAAAAGAG

FLAG-tag construct

• GP1405 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

References

• 15333955,34846166