priA

168

primosomal replication factor Y, part of the [wiki|replisome], required for survival of DNA damage

locus: BSU_15710

Molecular weight: 91.16 kDa

pI: 8.28

Protein length: 805 aa

Gene length: 2418 bp

function: [category|SW.3.1.1|DNA replication] initiation

product: primosomal replication factor Y

essential: yes

ec: 3.6.4.-

synonyms: priA, yloJ

List of homologs in different organisms, belongs to COG1198 (Galperin et al., 2021)

Gene

Coordinates: 1,644,068 → 1,646,485

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• binds stalled replication forks and initiates fork restart allowing for chromosomes to be fully duplicated under stressful conditions [pubmed|35007156]
• binds to abasic DNA lesions [pubmed|37250769]

Protein family

• [wiki|helicase family] (according to UniProt)

[wiki|Domains]

• [wiki|Helicase ATP-binding domain] (aa 282-448) (according to UniProt)
• [wiki|Helicase C-terminal domain] (aa 545-699) (according to UniProt)

Structure

• [PDB|4NL4] (from ''Klebsiella pneumoniae'', 38% identity) [Pubmed|24379377]
• [AF|P94461]

Expression and Regulation

Operons

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

References

Reviews

• 28320884,38052445

Original Publications

• 17853894,20122408,11585815,10572298,24379377,26883633,27484483,35007156,37250769