cysP

168

sulfate transport in via proton symport

locus: BSU_15580

Molecular weight: 36.68 kDa

pI: 10.27

Protein length: 354 aa

Gene length: 1065 bp

function: sulfate uptake

product: sulfate transport in via proton symport

essential: no

ec: null

synonyms: cysP, ylnA

List of homologs in different organisms, belongs to COG0306 (Galperin et al., 2021)

Gene

Coordinates: 1,631,095  1,632,159

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The protein

Protein family

• inorganic phosphate transporter (PiT) (TC 2.A.20) family (with [protein|BBFA6A5EE16CE6203AF89573F87678395277C9E8|pit], according to UniProt)

Structure

• [AF|O34734]

[wiki|Localization]

• cell membrane

Expression and Regulation

Operons

• genes: [gene|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]-[gene|58A5A7F12664EC38AC21E24C1AD89671D7E648CF|cysP]-[gene|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat]-[gene|3C71659E4868744A9301C26608EABF7B98217DCF|cysC]-[gene|92831D5A8E07A18BA6C6978123769D00D685BB45|ylnD]-[gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]-[gene|8BAB9B12D9D58A45446DC6CB7F48869089800FD3|ylnF]

  description: [Pubmed|11004190]

  regulation

  • induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
  • the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: transcription repression, [pubmed|], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
  • S-box: RNA switch, , the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11004190], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B365 (ylnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1364 NBRP B. subtilis, Japan]
• BKE15580 ([gene|58A5A7F12664EC38AC21E24C1AD89671D7E648CF|cysP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGCTAATTCCATTTTGCAGC,  downstream forward: _UP4_TGATCATTAATCTGAGTAAT
• BKK15580 ([gene|58A5A7F12664EC38AC21E24C1AD89671D7E648CF|cysP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGCTAATTCCATTTTGCAGC,  downstream forward: _UP4_TGATCATTAATCTGAGTAAT

labs

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

• 16267287,18039762,12107147