mraY

168

phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate), catalyzes the first commited membrane-bound step of bacterial peptidoglycan synthesis leading to the formation of lipid I

locus: BSU_15190

Molecular weight: 35.44 kDa

pI: 8.97

Protein length: 324 aa

Gene length: 975 bp

function: peptidoglycan precursor biosynthesis

product: phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate)

essential: yes

ec: 2.7.8.13

synonyms: mraY

List of homologs in different organisms, belongs to COG0472 (Galperin et al., 2021)

Gene

Coordinates: 1,587,926  1,588,900

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine --> Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + UMP (according to UniProt)

Protein family

• glycosyltransferase 4 family (with [protein|D9257531C2E24426781342314953B89A8272C5E5|tagO], according to UniProt)

Structure

• [PDB|5JNQ] [Pubmed|28068312]
• [AF|Q03521]

[wiki|Localization]

• cell membrane (according to UniProt)
• forms banded patterns along the length of the cell, this pattern formation depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [Pubmed|20233306]

Expression and Regulation

Operons

• genes: [gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]-[gene|4D3D4429E1F387BACB0D5A1084AA8DF179A16685|mraY]-[gene|0A9FF5A66F73464A780914A1424979CE753B3D9C|murD]-[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]

  description: [Pubmed|1391053]

  regulation

  • expressed during vegatative growth [Pubmed|1391053]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8320223], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

FLAG-tag construct

• GP2001 ''mraY-3xFLAG ermC'' (based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 36411352

Original Publications

• 15131133,8436954,18791230,18081839,7734839,21937437,18672909,20537545,10790316,1391053,24895118,25127465,26620564,20233306,27226570,27312048,27531195,28068312,30395652