moeB

168

molybdopterin biosynthesis protein

locus: BSU_14270

Molecular weight: 37.36 kDa

pI: 6.09

Protein length: 339 aa

Gene length: 1020 bp

function: nitrate respiration

product: unknown

essential: no

ec: null

synonyms: moeB

List of homologs in different organisms, belongs to COG0476 (Galperin et al., 2021)

Gene

Coordinates: 1,496,155 → 1,497,174

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The protein

Catalyzed reaction/ biological activity

• [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H⁺ --> [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate (according to UniProt)

Protein family

• HesA/MoeB/ThiF family (with [protein|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF] and [protein|37DA3A036937660AA750065B46B581D911D73D00|yrvM], according to UniProt)

Structure

• [PDB|1ZFN] (ThiF from ''E. coli'', 35% identity, 48% similarity) [Pubmed|15896804]
• [AF|O31702]

Paralogous protein(s)

• [protein|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]

Expression and Regulation

Operons

• genes: [gene|C6DB8499752ED1DBF276C0E46CC561450BED6A40|mobA]-[gene|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB]-[gene|03DBD906E31D6A8A4D14C9E2DACF0F1AB9F8DAA8|moeA]-[gene|A283C34375EDF495940E2F03C4FB6A9FE841FA1E|mobB]-[gene|12F27FA4DB8232B52271565C4F4937520A4F5C7B|moaE]-[gene|5C3EAEE1287FFB1541A31B31A8B6627E60A4D2EB|moaD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE14270 (Δ[gene|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAACCTGTTCTCCTTA,  downstream forward: _UP4_CTGTAACAACAGGAGGGGTC
• BKK14270 (Δ[gene|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAAACCTGTTCTCCTTA,  downstream forward: _UP4_CTGTAACAACAGGAGGGGTC

References

Reviews

• 23539623

Original Publications

• 15896804,22383849