ykuR

168

N-acetyl-diaminopimelate deacetylase

locus: BSU_14190

Molecular weight: 41.40 kDa

pI: 5.1

Protein length: 374 aa

Gene length: 1125 bp

function: biosynthesis of lysine and peptidoglycan

product: N-acetyl-diaminopimelate deacetylase

essential: yes

ec: 3.5.1.47

synonyms: ykuR, dapL

List of homologs in different organisms, belongs to COG1473 (Galperin et al., 2021)

Gene

Coordinates: 1,489,753 → 1,490,877

Phenotypes of a mutant

• essential [Pubmed|24261876,12682299]
• depletion of DapI results in swelling and rounding up of cells prior to lysis [Pubmed|24261876]

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The protein

Catalyzed reaction/ biological activity

• N-acetyl-LL-2,6-diaminopimelate + H₂O -→ acetate + LL-2,6-diaminopimelate [Pubmed|24261876]

Protein family

• [wiki|peptidase M20A family] (according to UniProt)

Structure

• [PDB|1YSJ] ([protein|C99AA3FDBB561BCED60915078DC746690C071753|sndB], 36% identity)
• [AF|O34916]

Paralogous protein(s)

• [protein|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA], [protein|C99AA3FDBB561BCED60915078DC746690C071753|sndB], [protein|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC]

[wiki|Localization]

• forms uniformly distributed foci in the cytoplasm [Pubmed|24261876]

Expression and Regulation

Operons

• genes: [gene|716FE06124544C05CC34BA2E643B4DBD27F04998|ykuQ]-[gene|D6F1906CE8386F78F451D6B794F74DE4AD25AC9C|ykuR]-[gene|3E7E0342AA86AFCC95F5CD5BEA5B10166DCE2721|ykuS]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B341 (ykuR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1340 NBRP B. subtilis, Japan]

References

• 24261876