mhqR

168

[wiki|MarR family ]transcriptional repressor of [gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2], [gene|7334017333600B876030056BE9247AD4E8996A1D|mhqA], [gene|43CD556065D2EF14365C78E014B7B2051CA0C78F|mhqD]-[gene|A1CB3DCF2EC78B9B0585CA6AC6F5BB37B8AF0208|mhqE]and [gene|A0842DD5F1B13AC15C95F18751E7836DF31B262A|mhqN]-[gene|ACAFB2072E74CC059B492A31EF492028B96C569F|mhqO]-[gene|search|mhqP ]expression

locus: BSU_13670

Molecular weight: 16.43 kDa

pI: 6.42

Protein length: 145 aa

Gene length: 438 bp

function: regulation of resistance to quinones and diamide

product: [wiki|MarR family ]transcriptional repressor

essential: no

synonyms: mhqR, ykvE

List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)

Gene

Coordinates: 1,433,199  1,433,636

Phenotypes of a mutant

• resistant to quinones and diamide
• inactivation of ''mhqR'' predisposes cells to grow without a wall (due to protection from oxidative stress) [Pubmed|26051891]

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The protein

[wiki|Domains]

• [wiki|HTH marR-type domain] (aa 5-137) (according to UniProt)

Structure

• [PDB|3GFJ] (from Sulfolobus tokodaii, 26% identity) [pubmed|19509310]
• [AF|O31672]

Paralogous protein(s)

• [protein|A703CA58365926CFE6BFC1C4A0B1DA22C67C85AC|ykoM]

Additional information

• Binding site: tATCTcgaAtTCgAGATaaaa [Pubmed|17725564]
• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000225 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|997B828A99D6D8460712C18ED0CE566B285C22DC|mhqR]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE13670 ([gene|997B828A99D6D8460712C18ED0CE566B285C22DC|mhqR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCAATCATCCTTTT,  downstream forward: _UP4_TAAAAAAAGGAAGCCTGATA
• BKK13670 ([gene|997B828A99D6D8460712C18ED0CE566B285C22DC|mhqR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTCAATCATCCTTTT,  downstream forward: _UP4_TAAAAAAAGGAAGCCTGATA

labs

• [wiki|Haike Antelmann],Free University of Berlin, Germany

References

• 18282125,26051891,17725564,22383849,27965289,19509310,34097790