thiW

168

thiamine [wiki|ECF transporter] (ATP-binding protein)

locus: BSU_13220

Molecular weight: 54.48 kDa

pI: 5.71

Protein length: 547 aa

Gene length: 1644 bp

function: thiamine uptake

product: thiamine [wiki|ECF transporter] (ATP-binding protein)

essential: no

synonyms: thiW, ykoD

List of homologs in different organisms, belongs to COG1123 (Galperin et al., 2021)

Gene

Coordinates: 1,388,809 → 1,390,452

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• 2 [wiki|ABC transporter domain]s (aa 8-250, aa 295-523) (according to UniProt)

Structure

• [PDB|4HUQ] (folate transporter from Lactobacillus brevis, 34% identity) [pubmed|23584589]
• [AF|O34362]

[wiki|Localization]

• membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]) [Pubmed|16291685]

Expression and Regulation

Operons

• genes: [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]

  description: [Pubmed|16291685]

  regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• MGNA-A755 (ykoD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/755 NBRP B. subtilis, Japan]
• BKE13220 (Δ[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA,  downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT
• BKK13220 (Δ[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA,  downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT

References

• 10092453,16291685,12376536,23584589