proB

proB
168

glutamate 5-kinase

locus
BSU_13120
Molecular weight
39.30 kDa
pI
6.01
Protein length
Gene length
function
biosynthesis of proline
product
glutamate 5-kinase
essential
no
ec
2.7.2.11
synonyms
proB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0263 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,378,496  1,379,593
Phenotypes of a mutant
auxotrophic for proline [Pubmed|21784929]
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)
Protein family
glutamate 5-kinase family (with [protein|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ], according to UniProt)
[wiki|Domains]
PUA domain (aa 276-353) (according to UniProt)
Structure
[PDB|2J5V] (from E. coli, 40% identity) [pubmed|17321544]
[AF|P39820]
Effectors of protein activity
the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}
Paralogous protein(s)
[protein|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]-[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]
description
[Pubmed|21233158]
regulation
induced by proline limitation ([wiki|T-box]) [Pubmed|21233158]
expression requires functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
T-box: anti-termination, in [regulon|other_regulator:T-box|T-box]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21233158], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
[protein|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA] is absent from the membrane proteome of a '[protein|A01637E1BFABA20A2923BA85A25CCD8A0D665F78|bdbC]-[protein|CED818CEF5B573A40F382157375E60C0B331F098|bdbD]' mutant [PubMed|22540663]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
Open in new tab

[gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]→[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]

2024-03-16 19:27:08

Jstuelk

55

a498fd63a6f2f5e81db95fe3ac7abe7368864c98

75EDCA3FF56BE0FC681BC2D3D6B71BE06F829994

Biological materials
Mutant
BKE13120 ([gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCTCCTCCGCGGC,  downstream forward: _UP4_GTAAAAGACTAGGAGGCGGA
BKK13120 ([gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCTCCTCCGCGGC,  downstream forward: _UP4_GTAAAAGACTAGGAGGCGGA
References
Reviews
25367752
Original Publications
19258532,16958849,17562291,21233158,21784929,28752945,17321544,29794222,30355672

5592C5163CF024289B850916516E2C8C7AD43136

Page visits: 2285

Time of last update: 2024-03-18 18:07:32

Author of last update: Melvin.boenninger