steT

168

serine/ threonine exchanger transporter

locus: BSU_12860

Molecular weight: 46.99 kDa

pI: 9.18

Protein length: 438 aa

Gene length: 1317 bp

function: exchange of serine and threonine

product: serine/ threonine exchanger transporter

essential: no

synonyms: steT, ykbA

List of homologs in different organisms, belongs to COG0531 (Galperin et al., 2021)

Gene

Coordinates: 1,351,375 → 1,352,691

Advertisement

The protein

Protein family

• [wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Structure

• [PDB|6F34] (from Geobacillus kaustophilus, 25% identity) [pubmed|29416041]
• [AF|O34739]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]

  description: [Pubmed|22383849]

  regulation

  • expression activated by glucose (4.2 fold) [Pubmed|12850135]

  regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [pubmed|34967415], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]

Biological materials

Mutant

• GP2378 ∆''[gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]'' ::''cat'', available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]
• GP2945 ∆''[gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]'' ::''kan'', available in [wiki|Jörg Stülke]'s lab
• MGNA-A736 (ykbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/736 NBRP B. subtilis, Japan]
• BKE12860 (Δ[gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG,  downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
• BKK12860 (Δ[gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG,  downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

lacZ fusion

• pGP2275 (in [wiki|pAC5]) (GP2962), available in [wiki|Jörg Stülke]'s lab

References

• 17344220,12850135,19419962,20610400,26976827,29416041,34967415