spxH

168

adaptor protein for [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-catalyzed [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] degradation, confers resistance against nitrosating agents

locus: BSU_11550

Molecular weight: 31.33 kDa

pI: 5.21

Protein length: 299 aa

Gene length: 900 bp

function: stimulation of [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] degradation

product: adaptor protein

essential: no

synonyms: yjbH

List of homologs in different organisms, belongs to COG2761 (Galperin et al., 2021)

Gene

Coordinates: 1,233,614  1,234,513

Phenotypes of a mutant

• increased thermotolerance due to increased stabiliy of [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] and thus increased expression of ''[gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]'' [Pubmed|24417481]
• defective in [category|SW.4.1.4|Swarming] motility (periodic cessation and reinitiation of [category|SW.4.1.4|Swarming] motility, terracing colonies) [pubmed|35638827]

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The protein

Catalyzed reaction/ biological activity

• adaptor protein for [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-catalyzed [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] degradation [Pubmed|19074380]

Protein family

• UPF0413 family (single member, according to UniProt)

[wiki|Cofactors]

• contains Zn atoms (coordinated by the N-terminal His-rich region) [Pubmed|19074380]

Structure

• [PDB|6GHB] (oxidized Geobacillus kaustophilus protein complexed with B. subtilis Spx) [Pubmed|30982633]
• [PDB|6GHO] (Geobacillus kaustophilus protein complexed with B. subtilis Spx) [Pubmed|30982633]
• [AF|O31606]

Effectors of protein activity

• [protein|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH] aggregates under stress conditions, resulting in the inability to bind to [protein|2C6386E9A63F410558D168798D077DF91590F454|spx], and therefore in stabilization of [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] [Pubmed|37330636,25353645]
• Zn atom is released upon treatment with strong oxidants [Pubmed|19074380]
• interaction with [protein|5EC57A51EF2DC48070615653B7A3C1007CFEE01D|yirB] inhibits the formation of a complex with [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] [Pubmed|21378193]

[wiki|Localization]

• cytosolic protein
• aggregates localize at nucleoid-free regions of the cell [pubmed|37330636]

Expression and Regulation

Operons

• genes: [gene|948BD57CC45915C7CDDB8EF168287533FC514B7C|yjbI]-[gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH]

  description: [Pubmed|17293416]

  regulation

  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|spx]) [pubmed|29271514]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|29271514], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between [gene|138DC46C976BAA18893B70DF040BA1BB2FA0A77C|yizD] and [gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH] [PubMed|20525796]

Biological materials

Mutant

• GP2645 ([gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH]::''erm''), available in [wiki|Jörg Stülke]'s lab
• BKE11550 (''[gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE11550 ([gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTATAGCTCATGCTGATAGT,  downstream forward: _UP4_TAGCCGCAGGCGTGCATATG
• BKK11550 ([gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTATAGCTCATGCTGATAGT,  downstream forward: _UP4_TAGCCGCAGGCGTGCATATG

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP3799 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Peter Zuber], Oregon Health and Science University, USA
• [http://www.ogi.edu/people/dsp_person.cfm?person_id=411D6801-2A56-D16D-58A06B4480EDB9C7 Homepage]
• [wiki|Claes von Wachenfeldt], Lund University, Sweden [http://aron.ldc.lu.se/kundwebb/cellorg/mibiol/research/wachen/index.htm Homepage]

References

Reviews

• 23375660,23479438,19609260,28748186

Original Publications

• 17293416,19074380,17908206,20525796,21378193,21947404,24417481,24942655,25353645,27191337,29271514,30982633,34181483,35638827,37330636