oppA

168

oligopeptide [wiki|ABC transporter] (binding protein)

locus: BSU_11430

Molecular weight: 61.32 kDa

pI: 5.72

Protein length: 545 aa

Gene length: 1638 bp

function: initiation of [wiki|sporulation], competence development

product: oligopeptide [wiki|ABC transporter] (binding protein)

essential: no

synonyms: oppA, spo0KA

List of homologs in different organisms, belongs to COG4166 (Galperin et al., 2021)

Gene

Coordinates: 1,219,849  1,221,486

Phenotypes of a mutant

• reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]

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The protein

Catalyzed reaction/ biological activity

• required for the uptake of quorum sensing peptides such as [protein|4FA7D6E6316A6FC71C10C692D125833263894020|phrH] [Pubmed|21908671]

Protein family

• [wiki|bacterial solute-binding protein 5 family] (according to UniProt)

Structure

• [PDB|8ARN] (complex with SNSS peptide) [pubmed|36748525]
• [PDB|8ARE] (complex with [protein|997434F9679B3A2F8D2453D0C3BAB24F5BB90922|phrE] peptide) [pubmed|36748525]
• [PDB|1OLA] (from Salmonella enterica, 36% identity) [pubmed|8202710]
• [AF|P24141]

Modification

• phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 [Pubmed|17218307]

Paralogous protein(s)

• [protein|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]

[wiki|Localization]

• attached to the cell membrane (via [protein|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[protein|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]) [Pubmed|10092453]
• at the outer side of the cell: extracellular (signal peptide) [Pubmed|18957862],  lipid modification as retention signal [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]

  description: [Pubmed|1901616]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, no derepression occurs, however, in a [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] mutant, due to increased repression by [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC] [Pubmed|25966844], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|10383984], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|trpS]' and '[protein|search|oppA]' [PubMed|20525796]

Biological materials

Mutant

• BP67 (spc) available in [wiki|Fabian Commichau]'s lab
• 1S118 ( ''oppA''::''spec''), [Pubmed|11267663], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S118&Search=1S118 BGSC]
• GP2097 (D(''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]'')::''aphA3''), available in [wiki|Jörg Stülke]'s lab
• BKE11430 (''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE11430 ([gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCGCAAACCCCCTAA,  downstream forward: _UP4_TAAGGCTACGTCTGAAAATA
• BKK11430 ([gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCGCAAACCCCCTAA,  downstream forward: _UP4_TAAGGCTACGTCTGAAAATA

References

• 36815589,1901616,10092453,27231947,11267663,9252573,10960106,12823818,10383984,18957862,18763711,17218307,7997159,20525796,23651456,21908671,25755103,25966844,1899858,23199363,8202710,31426791,36574412,36748525,37755230