appB

168

oligopeptide [wiki|ABC transporter] (permease)

locus: BSU_11390

Molecular weight: 35.25 kDa

pI: 9.77

Protein length: 317 aa

Gene length: 951 bp

function: uptake of oligopeptides

product: oligopeptide [wiki|ABC transporter] (permease)

essential: no

synonyms: appB

List of homologs in different organisms, belongs to COG0601 (Galperin et al., 2021)

Gene

Coordinates: 1,215,243 → 1,216,193

Advertisement

The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
• [wiki|OppBC subfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transmembrane type-1 domain] (aa 96-303) (according to UniProt)

Structure

• [AF|P42062]

Paralogous protein(s)

• [protein|062AE5FB4C778178A7F6833CB8BD9D631E461E89|dppB], [protein|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|325BCDCAB4003204294229B7F9F0A5F353D72B54|appD]-[gene|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF]-[gene|DD2C5F549759447FF1C30E5B43BCB74CE4320EF3|appA/1]-[gene|082D2012ABE3671F62211F77ED501849BF77B4EF|appA/2]-[gene|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|appB]-[gene|252E3F0A05D78CEC46A08B46D1C6133239B6B8BA|appC]

  description: [Pubmed|7997159]

  regulation

  • repressed by [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] [Pubmed|12618455]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|25755103], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455] [pubmed|18083814], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|10383984], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]

Biological materials

Mutant

• GP2100 (D(''[gene|325BCDCAB4003204294229B7F9F0A5F353D72B54|appD]-[gene|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF]-[gene|082D2012ABE3671F62211F77ED501849BF77B4EF|appA/2]-[gene|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|appB]-[gene|252E3F0A05D78CEC46A08B46D1C6133239B6B8BA|appC]'')::''spc''), available in [wiki|Jörg Stülke]'s lab
• BKE11390 (Δ[gene|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|appB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11390 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATACTTGTCCCCCTTTA,  downstream forward: _UP4_GAATATTAAAAGGAGGGAAA
• BKK11390 (Δ[gene|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|appB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11390 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATACTTGTCCCCCTTTA,  downstream forward: _UP4_GAATATTAAAAGGAGGGAAA

References

• 12618455,7997159,10092453,10383984,12618455,12107147,25755103,37755230