hlpB

168

HNH nuclease-like protein, rescues [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-mediated recombination intermediates

locus: BSU_10660

Molecular weight: 11.31 kDa

pI: 9.94

Protein length: 100 aa

Gene length: 303 bp

function: rescuing of [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-mediated recombination intermediates

product: HNH nuclease-like protein

essential: no

synonyms: hlpB, yisB

List of homologs in different organisms

Gene

Coordinates: 1,148,155  1,148,457

Phenotypes of a mutant

• essential, the mutation can be suppressed by second-site mutations in [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA] or [gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB] [Pubmed|22984257], non-essential according to [Pubmed|28189581]

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The protein

Structure

• [AF|O06715]

[wiki|Localization]

• evenly distributed throughout the cytosol in growing cells [Pubmed|22984257]
• forms a single focus on the nucleoid upon induction of DNA damage [Pubmed|22984257]

Expression and Regulation

Operons

• genes: [gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-[gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]-[gene|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|sbcC]-[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]

  description: [Pubmed|22383849]

  regulation

  • positive control by [protein|search|ComK] [Pubmed|7746142]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7746142], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• GP3559 (Δ[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]::kan), available in [wiki|Jörg Stülke]'s lab
• MGNA-A503 (yisB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/503 NBRP B. subtilis, Japan]
• BKE10660 ([gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC,  downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
• BKK10660 ([gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC,  downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT

References

• 11948146,22984257,22383849,28189581