hemY

168

coproporphyrinogen oxidase

locus: BSU_10140

Molecular weight: 51.04 kDa

pI: 8.04

Protein length: 470 aa

Gene length: 1413 bp

function: biosynthesis of heme

product: coproporphyrinogen oxidase

essential: no

ec: 1.3.3.4

synonyms: hemY, hemG, cgoX

List of homologs in different organisms, belongs to COG1232 (Galperin et al., 2021)

Gene

Coordinates: 1,088,197  1,089,609

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The protein

Catalyzed reaction/ biological activity

• coproporphyrinogen --> coproporphyrin [pubmed|25646457]
• 3 O₂ + protoporphyrinogen IX --> 3 H₂O₂ + protoporphyrin IX (according to UniProt)

Protein family

• Protoporphyrinogen oxidase family (single member, according to UniProt)

[wiki|Cofactors]

• FAD [pubmed|37102418]

Structure

• [PDB|3I6D]
• [AF|P32397]

[wiki|Localization]

• cell membrane (peripheral) [pubmed|7928957]

Expression and Regulation

Operons

• genes: [gene|762E7731EFEAC9F38074D7A7E9A3E5759437CDCA|hemE]-[gene|E4C588EC93132CCBE4F40559F8A480A66A2D82D7|hemH]-[gene|7541AD7D06BC4CE30AD576562E0053CC1E25B4D6|hemY]

  description: [Pubmed|1459957]

Biological materials

Mutant

• BKE10140 ([gene|7541AD7D06BC4CE30AD576562E0053CC1E25B4D6|hemY]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCACTCATGTTCATCGCCT,  downstream forward: _UP4_TAAAACCTCCGCTTTATCGC
• BKK10140 ([gene|7541AD7D06BC4CE30AD576562E0053CC1E25B4D6|hemY]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCACTCATGTTCATCGCCT,  downstream forward: _UP4_TAAAACCTCCGCTTTATCGC

References

Reviews

• 28123057,37593721

Original Publications

• 9217019,1459957,7928957,19266155,19944166,22273380,25646457,8288631,28739897,37102418,36815589