hemH

168

coproporphyrin ferrochelatase

locus: BSU_10130

Molecular weight: 35.19 kDa

pI: 4.62

Protein length: 310 aa

Gene length: 933 bp

function: biosynthesis of heme

product: coproporphyrin ferrochelatase

essential: no

ec: 4.99.1.1

synonyms: hemH, hemF, cpfC

List of homologs in different organisms, belongs to COG0276 (Galperin et al., 2021)

Gene

Coordinates: 1,087,250 → 1,088,182

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The protein

Catalyzed reaction/ biological activity

• coproporphyrin --> coproheme [pubmed|25646457]
• 2 H⁺ + heme b --> Fe²⁺ + protoporphyrin IX (according to UniProt)

Protein family

• ferrochelatase family (with [protein|8BAB9B12D9D58A45446DC6CB7F48869089800FD3|ylnF], according to UniProt)

Structure

• [PDB|2HK6] (complex with iron) [pubmed|17198378]
• [PDB|1AK1] [pubmed|9384565]
• [AF|P32396]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|762E7731EFEAC9F38074D7A7E9A3E5759437CDCA|hemE]-[gene|E4C588EC93132CCBE4F40559F8A480A66A2D82D7|hemH]-[gene|7541AD7D06BC4CE30AD576562E0053CC1E25B4D6|hemY]

  description: [Pubmed|1459957]

Biological materials

Mutant

• BKE10130 (Δ[gene|E4C588EC93132CCBE4F40559F8A480A66A2D82D7|hemH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCCTCTTTA,  downstream forward: _UP4_GGACGTTAAAGAAGGCGATG
• BKK10130 (Δ[gene|E4C588EC93132CCBE4F40559F8A480A66A2D82D7|hemH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCCTCTTTA,  downstream forward: _UP4_GGACGTTAAAGAAGGCGATG

References

Reviews

• 28123057,37593721

Original Publications

• 9384565,1459957,8119288,21052751,23097001,16453119,25826316,28864672,17198378,9384565,12761666,10704318,25646457