recX

recX
168

recombination protein, modulates the SOS response and facilitates [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-mediated recombinational repair and genetic recombination, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation

locus
BSU_08520
Molecular weight
30.86 kDa
pI
6.29
Protein length
Gene length
function
[wiki|DNA repair/ recombination]
product
recombination protein
essential
no
synonyms
recX, yfhG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2137 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
925,633  926,427
Phenotypes of a mutant
reduced natural transformation with plasmid or chromosomal DNA [Pubmed|23284295]
drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
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The protein
Catalyzed reaction/ biological activity
modulates the "length or packing" of a [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] filament, thereby [protein|144E7D10740C5F146403612B8A0C88E02FB9C572|recX] facilitates the initiation of recombination and increases recombination across species [Pubmed|23284295]
inhibits [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-catalyzed ATP hydrolysis [pubmed|28911099]
inhibits stable [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleation and polymerization on ssDNA [pubmed|28911099]
induces [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleoprotein filament depolymerization [pubmed|28911099]
promotes a net [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly from viral ssDNAs not homologous to the host genome [pubmed|31876108]
Protein family
recX family (single member, according to UniProt)
Structure
[PDB|3D5L] (from Lactobacillus reuterii, 36% identity)
[AF|O31575]
[wiki|Localization]
forms foci on the nucleoid upon DNA damage [Pubmed|23284295]
forms foci at the cells poles and/ or the nucleoid upon induction of genetic competence [Pubmed|23284295]
Expression and Regulation
Operons
genes
[gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]-[gene|CEB1A0835C26E14E04BA99F933787C4550A96388|yfhH]
description
[Pubmed|22383849]
Open in new tab

[gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]→[gene|CEB1A0835C26E14E04BA99F933787C4550A96388|yfhH]

2024-02-09 23:39:54

ghost

43

5b6a385ee43aaac4edfea85d0c0679b53a109b6a

E4A7EA8284350823BE195BBA357E25294134B7CF

Biological materials
Mutant
GP3533 (Δ[gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-C361 (yfhG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2359 NBRP B. subtilis, Japan]
BKE08520 ([gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTAATCTCCCTTCTCA,  downstream forward: _UP4_TTACTGCAGGAAGAGGAGTA
BKK08520 ([gene|144E7D10740C5F146403612B8A0C88E02FB9C572|recX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08520 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_CATCTTAATCTCCCTTCTCA,  downstream forward: _UP4_TTACTGCAGGAAGAGGAGTA
References
23284295,22383849,24285298,24285298,28911099,30050509,31876108,33264457,38052445

144E7D10740C5F146403612B8A0C88E02FB9C572

Page visits: 3689

Time of last update: 2024-03-29 02:02:33

Author of last update: Jstuelk