yfiC

168

similar to [wiki|ABC transporter] (ATP-binding protein)

locus: BSU_08220

Molecular weight: 67.22 kDa

pI: 9.62

Protein length: 604 aa

Gene length: 1815 bp

function: unknown

product: [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: yfiC

List of homologs in different organisms, belongs to COG1132 (Galperin et al., 2021)

Gene

Coordinates: 895,619 → 897,433

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
• [wiki|ABC transmembrane type-1 domain] (aa 49-332) (according to UniProt)
• [wiki|ABC transporter domain] (aa 366-600) (according to UniProt)

Structure

• [PDB|3QF4] ([protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]-[protein|E85814EDF232D21A42380D559EAD2CDFB41F19DB|yfiC] complex from Thermotoga maritima, 48% identity) [pubmed|22447242]
• [AF|P54719]

Paralogous protein(s)

• [protein|ED1F82463BAA28B67184BFAB5CB23CF26A62999D|bmrA], [protein|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|yknU], [protein|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV], [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|ygaD], [protein|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA], [protein|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC], [protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|yfiB]-[gene|E85814EDF232D21A42380D559EAD2CDFB41F19DB|yfiC]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C293 (yfiC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2291 NBRP B. subtilis, Japan]
• BKE08220 (Δ[gene|E85814EDF232D21A42380D559EAD2CDFB41F19DB|yfiC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGGAAAGGCTTGCGGATGT,  downstream forward: _UP4_TAGACCTTTGTCCTGCACAG
• BKK08220 (Δ[gene|E85814EDF232D21A42380D559EAD2CDFB41F19DB|yfiC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGGAAAGGCTTGCGGATGT,  downstream forward: _UP4_TAGACCTTTGTCCTGCACAG

References

• 10092453,22447242