fecF

168

iron/citrate ABC transporter (ATP-binding protein)

locus: BSU_07490

Molecular weight: 29.31 kDa

pI: 6.07

Protein length: 266 aa

Gene length: 801 bp

function: iron uptake

product: iron/citrate ABC transporter (ATP-binding protein)

essential: no

ec: 3.6.3.-

synonyms: fecF, yfmF

List of homologs in different organisms, belongs to COG1120 (Galperin et al., 2021)

Gene

Coordinates: 822,903 → 823,703

Advertisement

The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 4-240) (according to UniProt)

Structure

• [PDB|4G1U] (from ''Yersinia pestis'', the [protein|BDAA56484E05DA3C1CB0CA09873A365657999679|fecD]-[protein|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]-[protein|E76640F895261DA34AD2342B54B43D11857AEA9A|fecF] complex, 32% identity) [Pubmed|23142986]
• [AF|O34510]

Paralogous protein(s)

• [protein|E70BE74D5AB4B06CDFF902E58A0B9EBF477E21EC|fhuC], [protein|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP], [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV]

[wiki|Localization]

• associated to the membrane (via [protein|BDAA56484E05DA3C1CB0CA09873A365657999679|fecD]-[protein|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|569510093E5B69A0368205920A21A81CADF030E9|fecC]-[gene|BDAA56484E05DA3C1CB0CA09873A365657999679|fecD]-[gene|800EAB1D8617589CCAC68FB874EFCDE4C77962E1|fecE]-[gene|E76640F895261DA34AD2342B54B43D11857AEA9A|fecF]

  description: [Pubmed|16672620]

  regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,16672620,12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [Pubmed|16672620,12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

Biological materials

Mutant

• MGNA-C244 (yfmF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2242 NBRP B. subtilis, Japan]
• BKE07490 (Δ[gene|E76640F895261DA34AD2342B54B43D11857AEA9A|fecF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAGTTTTCCCATGGTATCCT,  downstream forward: _UP4_TGAAAAGGAGAGGAATTCCC
• BKK07490 (Δ[gene|E76640F895261DA34AD2342B54B43D11857AEA9A|fecF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAGTTTTCCCATGGTATCCT,  downstream forward: _UP4_TGAAAAGGAGAGGAATTCCC

References

• 10092453,16672620,23142986,29133393