yfmR

168

translation factor, ABCF ATPase, prevents ribosome stalling during early elongation in the absence of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]

locus: BSU_07370

Molecular weight: 70.94 kDa

pI: 5

Protein length: 629 aa

Gene length: 1890 bp

function: prevents ribosome stalling in the absence of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]

product: [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: yfmR

List of homologs in different organisms, belongs to COG0488 (Galperin et al., 2021)

Gene

Coordinates: 809,557 → 811,446

Phenotypes of a mutant

• an [gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] [gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR] double mutant is not viable [pubmed|38349882]

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The protein

Catalyzed reaction/ biological activity

• a paraloguous protein from ''E. coli''  (EttA) gates [wiki|ribosome] entry into the translation elongation cycle [Pubmed|24389465,24389466]

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)
• [wiki|ABCF ATPase subfamily] [pubmed|30597160]

[wiki|Domains]

• 2 [wiki|ABC transporter domain]s (aa 4-255, aa 319-537) (according to UniProt)

Structure

• [PDB|3J5S] (EttA from E. coli, 36% identity) [pubmed|24389465]
• [PDB|4FIN] (EttA from E. coli, 36% identity) [pubmed|24389466]
• [AF|O06476]

Paralogous protein(s)

• [protein|1F56D3275386BB54A774BFA045C09E36D1268471|ydiF], [protein|2F79DB15D359A127C2D833CC0465E18E1725F539|ykpA], [protein|47B644F334A0BD501E77A198A61CE0F22BAB3E5E|yfmM]

Expression and Regulation

Operons

• genes: [gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C234 (yfmR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2232 NBRP B. subtilis, Japan]
• BKE07370 (Δ[gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTTCCTATCAATCCTC,  downstream forward: _UP4_TAAAAAGCGTGGCCGCAGCA
• BKK07370 (Δ[gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTTCCTATCAATCCTC,  downstream forward: _UP4_TAAAAAGCGTGGCCGCAGCA

References

Reviews

• 30746819,24500425

Original Publications

• 10092453,24389466,24389465,27006457,38349882