yetO

168

cytochrome P450 (CYP102A2)/ NADPH-cytochrome P450 reductase

locus: BSU_07250

Molecular weight: 119.26 kDa

pI: 5.73

Protein length: 1061 aa

Gene length: 3186 bp

function: fatty acid metabolism

product: NADPH-cytochrome P450 reductase

essential: no

ec: 1.6.2.4

synonyms: yetO, yfnJ

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene

Coordinates: 792,682  795,867

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The protein

Catalyzed reaction/ biological activity

• oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids [Pubmed|15375636]
• alkane + O₂ + reduced [NADPH—hemoprotein reductase] --> primary alcohol + H⁺ + H₂O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
• NADPH + 2 oxidized [cytochrome P450] --> H⁺ + NADP⁺ + 2 reduced [cytochrome P450] (according to UniProt)

Protein family

• [wiki|cytochrome P450] family (according to UniProt)

[wiki|Domains]

• [wiki|Flavodoxin-like domain] (aa 493-632) (according to UniProt)
• [wiki|FAD-binding FR-type domain] (aa 671-904) (according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|2X7Y] (from ''Bacillus megaterium''; 66% identity, 87% similarity)  [Pubmed|25325618,22949185]
• [AF|O08394]

Paralogous protein(s)

• [protein|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C225 (yfnJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2223 NBRP B. subtilis, Japan]
• BKE07250 ([gene|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
• BKK07250 ([gene|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA

References

• 15122913,15375636,16716428,15857787,11734890,21048857,25325618,22949185