yerD

168

general stress protein, similar to glutamate synthase (ferredoxin), required for protection against paraquat stress

locus: BSU_06590

Molecular weight: 58.58 kDa

pI: 7.35

Protein length: 525 aa

Gene length: 1578 bp

function: protection against paraquat stress

product: unknown

essential: no

synonyms: yerD

List of homologs in different organisms, belongs to COG0069 (Galperin et al., 2021)

Gene

Coordinates: 716,780 → 718,357

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The protein

Protein family

• glutamate synthase family (with [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA] and [protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB], according to UniProt)

[wiki|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|1EA0] (from Azospirillum brasilense, corresponds to aa 184 ... 495, 30% identity)
• [AF|O34849]

Paralogous protein(s)

• [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A925 (yerD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/925 NBRP B. subtilis, Japan]
• GP1184 (tet) available in [wiki|Jörg Stülke]'s lab
• BKE06590 (Δ[gene|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|yerD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTCAGCCTCCCTTTT,  downstream forward: _UP4_TAAAGAAACCGCCCTTGCCA
• BKK06590 (Δ[gene|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|yerD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTCAGCCTCCCTTTT,  downstream forward: _UP4_TAAAGAAACCGCCCTTGCCA

Expression vectors

• pBP32 (expression vector for ''E. coli'' BL21; N-terminal Strep-tag-YerD fusion protein, based on [wiki|pGP172]), available in [wiki|Fabian Commichau]'s lab

FLAG-tag construct

• GP1187 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

References

• 15805528,22383849,23033921,22582280