purD

168

phosphoribosylglycinamide synthetase

locus: BSU_06530

Molecular weight: 45.12 kDa

pI: 4.6

Protein length: 422 aa

Gene length: 1269 bp

function: purine biosynthesis

product: phosphoribosylglycinamide synthetase

essential: no

ec: 6.3.4.13

synonyms: purD

List of homologs in different organisms, belongs to COG0151 (Galperin et al., 2021)

Gene

Coordinates: 710,148 711,416

Advertisement

The protein

Catalyzed reaction/ biological activity

• 5-phospho-D-ribosylamine + ATP + glycine --> ADP + H⁺ + N¹-(5-phospho-D-ribosyl)glycinamide + phosphate (according to UniProt)

Protein family

• GARS family (single member, according to UniProt)

[wiki|Domains]

• [wiki|ATP-grasp doamin] (aa 107-313) (according to UniProt)

Structure

• [PDB|2XCL]
• [AF|P12039]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE06530 ([gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG
• BKK06530 ([gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG

References

• 3036807,12923093,15378759,7638212