gmuB

168

glucomannan-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIB of the [category|SW.1.2.2|PTS]

locus: BSU_05810

Molecular weight: 11.30 kDa

pI: 4.72

Protein length: 103 aa

Gene length: 312 bp

function: glucomannan uptake and phosphorylation

product: glucomannan-specific lichenan-specific [category|SW.1.2.2|PTS], EIIB component

essential: no

synonyms: gmuB, ydhM

List of homologs in different organisms, belongs to COG1440 (Galperin et al., 2021)

Gene

Coordinates: 626,622  626,933

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The protein

Catalyzed reaction/ biological activity

• D-cellobiose + N^π-phospho-L-histidyl-[protein] --> 6-phospho-β-D-glucosyl-(1→4)-D-glucose + L-histidyl-[protein] (according to UniProt)

Protein family

• [category|SW.1.2.2|PTS] permease, lactose family [Pubmed|10627040]

[wiki|Domains]

• [wiki|PTS EIIB domain] type-3 (aa 1-103) (according to UniProt)

Structure

• [PDB|4MGE] (from B. anthracis, 46% identity)
• [AF|O05505]

Paralogous protein(s)

• [protein|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]

Biological materials

Mutant

• MGNA-C188 (ydhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2186 NBRP B. subtilis, Japan]
• BKE05810 ([gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT,  downstream forward: _UP4_TTGTCCTTAATGGTGAATCA
• BKK05810 ([gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT,  downstream forward: _UP4_TTGTCCTTAATGGTGAATCA

References

• 18177310,10627040,22900538,20817675