phoB

168

alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase

locus: BSU_05740

Molecular weight: 50.36 kDa

pI: 5.89

Protein length: 462 aa

Gene length: 1389 bp

function: aquisition of phosphate upon phosphate starvation, ether lipid synthesis

product: alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase

essential: no

ec: 3.1.3.1

synonyms: phoB

List of homologs in different organisms, belongs to COG1785 (Galperin et al., 2021)

Gene

Coordinates: 620,097  621,485

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The protein

Catalyzed reaction/ biological activity

• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• alkaline phosphatase family (with [protein|E0430C16C41440633647976A0F9183E24268E198|phoA], according to UniProt)

Structure

• [PDB|3A52] (from ''Shewanella sp.'', 41% identity) [Pubmed|20057143]
• [AF|P19405]

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Paralogous protein(s)

• [protein|E0430C16C41440633647976A0F9183E24268E198|phoA]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]-[gene|66D3E978E3DBA0410CA5597B5B860DF27C97335A|ydhF]

  description: [Pubmed|10913081]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|9335276,10913081,16030210]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|9335276,10913081,16030210], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,16030210], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• 1A767 ( ''phoB''::''cat''), [Pubmed|9426145], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A767&Search=1A767 BGSC]
• BKE05740 ([gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTGGATAACCTCCTAAA,  downstream forward: _UP4_TAAAAGAATACAAGGTGTCC
• BKK05740 ([gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTGGATAACCTCCTAAA,  downstream forward: _UP4_TAAAAGAATACAAGGTGTCC

References

• 16030210,9335276,8088554,10913081,16491025,8113174,9335276,18957862,16493705,25666134,20057143,27118079,27226549