dctB

168

C4-dicarboxylate binding protein, co-sensor for DctS

locus: BSU_04440

Molecular weight: 39.70 kDa

pI: 4.89

Protein length: 350 aa

Gene length: 1053 bp

function: sensing of C4-dicarboxylates

product: C4-dicarboxylate binding protein

essential: no

synonyms: dctB, ydbE

List of homologs in different organisms, belongs to COG1638 (Galperin et al., 2021)

Gene

Coordinates: 496,646  497,698

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The protein

Catalyzed reaction/ biological activity

• not involved in C4-dicarboxylate transport  [Pubmed|24375102]
• acts as co-sensor for [protein|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]  [Pubmed|24375102]

Protein family

• bacterial solute-binding protein 7 family (single member, according to UniProt)

Structure

• [PDB|4O94] (from Rhodopseudomonas palustris, 41% identity) [pubmed|25540822]
• [AF|P96600]

[wiki|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7428E80AC67A659B8602AF887EA7DFFD4976B40C|dctB]-[gene|01CE4BBBEB0C0855201D396FB8036F72B64EA7DD|ydbD]

  description: [Pubmed|10708364]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• BKE04440 ([gene|7428E80AC67A659B8602AF887EA7DFFD4976B40C|dctB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAAGCCCTCCCCTAT,  downstream forward: _UP4_TAGTTCCCATCTCGATACGC
• BKK04440 ([gene|7428E80AC67A659B8602AF887EA7DFFD4976B40C|dctB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAAGCCCTCCCCTAT,  downstream forward: _UP4_TAGTTCCCATCTCGATACGC

References

• 10708364,24375102,25540822