ycgK

168

similar to transcriptional regulator ([wiki|LysR family])

locus: BSU_03170

Molecular weight: 36.25 kDa

pI: 7.28

Protein length: 324 aa

Gene length: 975 bp

function: unknown

product: transcriptional regulator ([wiki|LysR family])

essential: no

synonyms: ycgK

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

Gene

Coordinates: 341,492 → 342,466

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The protein

Protein family

• [wiki|LysR family] (according to UniProt)

[wiki|Domains]

• [wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)

Structure

• [PDB|4X6G] (from Pseudomonas aeruginosa, 29% identity) [pubmed|25931525]
• [AF|P94387]

Expression and Regulation

Operons

• genes: [gene|D28190620D5B834DD7F42822B835C6FE932FD8A5|ycgK]-[gene|6BCE78F623A40B519211E79AFA1AD898318BD817|cah]-[gene|7306A55192AEB4032FD17A1BAAFF1127D511D70E|ycgL]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B990 (ycgK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1989 NBRP B. subtilis, Japan]
• BKE03170 (Δ[gene|D28190620D5B834DD7F42822B835C6FE932FD8A5|ycgK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCAGCCTCCATAGGTT,  downstream forward: _UP4_TAGAAAACAAAGACGAAACG
• BKK03170 (Δ[gene|D28190620D5B834DD7F42822B835C6FE932FD8A5|ycgK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCAGCCTCCATAGGTT,  downstream forward: _UP4_TAGAAAACAAAGACGAAACG

References

Research papers

• 25931525