nadE

168

NH3-dependent NAD+ synthetase

locus: BSU_03130

Molecular weight: 30.24 kDa

pI: 4.89

Protein length: 272 aa

Gene length: 819 bp

function: NAD biosynthesis

product: NH3-dependent NAD+ synthetase

essential: yes

ec: 6.3.1.5

synonyms: nadE, outB, tscBGH, gsp-81

List of homologs in different organisms, belongs to COG0171 (Galperin et al., 2021)

Gene

Coordinates: 338,288  339,106

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• ATP + deamido-NAD⁺ + NH₄⁺ --> AMP + diphosphate + H⁺ + NAD⁺ (according to UniProt)

Protein family

• NAD synthetase family (single member, according to UniProt)

Structure

• [PDB|1EE1] [pubmed|11375500]
• [AF|P08164]

Expression and Regulation

Operons

• genes: [gene|4B74B1653D783779AFB37EDF9D33E7D6F1AC92B5|nadE]

  description: [Pubmed|2435704]

  regulation

  • induced by antibiotics interacting with the cytoplasmic membrane [Pubmed|22926563]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|9271869,11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

References

• 9271869,8450306,19554628,11544224,22926563,11375500