pcp

168

pyrrolidone-carboxylate peptidase

locus: BSU_02650

Molecular weight: 23.63 kDa

pI: 6.42

Protein length: 215 aa

Gene length: 648 bp

function: removal of the N-terminal pyroglutamyl group from peptides

product: pyrrolidone-carboxylate peptidase

essential: no

ec: 3.4.11.8

synonyms: pcp

List of homologs in different organisms, belongs to COG2039 (Galperin et al., 2021)

Gene

Coordinates: 286,773  287,420

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The protein

Catalyzed reaction/ biological activity

• Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro (according to UniProt)

Protein family

• peptidase C15 family (single member, according to UniProt)

Structure

• [PDB|1AUG] (from B. amyloliquefaciens, 72% identity) [pubmed|10196127]
• [AF|P28618]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|13E80EAC33C30BB0BBF08A0F0E37EB18CC87C8CA|pcp]

  description: [pubmed|22383849]

Biological materials

Mutant

• BKE02650 ([gene|13E80EAC33C30BB0BBF08A0F0E37EB18CC87C8CA|pcp]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCATCTCCTTTTT,  downstream forward: _UP4_CACTAAGCGGCCGCCGCTGT
• BKK02650 ([gene|13E80EAC33C30BB0BBF08A0F0E37EB18CC87C8CA|pcp]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCATCTCCTTTTT,  downstream forward: _UP4_CACTAAGCGGCCGCCGCTGT

References

• 1362573,1353026,10196127