tatAD

168

component of the [protein|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD] twin-arginine translocase

locus: BSU_02630

Molecular weight: 7.37 kDa

pI: 9.69

Protein length: 70 aa

Gene length: 213 bp

function: TAT [wiki|protein secretion]

product: component of the twin-arginine translocation pathway

essential: no

synonyms: tatAD, yczB

List of homologs in different organisms, belongs to COG1826 (Galperin et al., 2021)

Gene

Coordinates: 285,775 → 285,987

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The protein

Catalyzed reaction/ biological activity

• translocation of [protein|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD] [Pubmed|19395490]

Protein family

• TatA/E family (with [protein|8987552EFDF673776741405F7F5FE004556484CA|tatAC] and [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY], according to UniProt)

Structure

• [PDB|2L16] [Pubmed|20726548]
• [AF|O31467]

Paralogous protein(s)

• [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY], [protein|8987552EFDF673776741405F7F5FE004556484CA|tatAC]

[wiki|Localization]

• membrane (according to Swiss-Prot),  forms foci at the division sites and cell poles, localization requires interaction with [protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD] or [protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY] [Pubmed|19395490]

Expression and Regulation

Operons

• genes: [gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]-[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[gene|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD]

  description: [Pubmed|22383849]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|10094677]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|10094677,11007775], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10094677], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • expression of the operon depends on functional [protein|search|HemAT] and [protein|search|CsbC] [PubMed|23180473]

Biological materials

Mutant

• BKE02630 (Δ[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTTGAAAACATAATTTCCA,  downstream forward: _UP4_TGAATGCTGATGAGGCAGAC
• BKK02630 (Δ[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTTGAAAACATAATTTCCA,  downstream forward: _UP4_TGAATGCTGATGAGGCAGAC

labs

• [wiki|Jan Maarten van Dijl], University of Groningen, The Netherlands, [https://www.rug.nl/staff/j.m.van.dijl/ Homepage]

References

Reviews

• 22683878,24140208,25975269,25494301,27121927,31401776

Original Publications

• 12867413,15554971,20726548,11007775,19395490,21479178,21479530,22383849,10094677,23180473,23567937,20977272,27984091,36523158