ndhF

168

subunit of a Na⁺:bicarbonate transporter [protein|CCDDC414E266D86B9027EA67EA565D87357023B3|MpsA]-[protein|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|MpsB]

locus: BSU_01830

Molecular weight: 55.30 kDa

pI: 10.15

Protein length: 505 aa

Gene length: 1518 bp

function: uptake of bicarbonate

product: subunit of a Na⁺:bicarbonate transporter

essential: no

synonyms: ndhF, ybxE, mpsA

List of homologs in different organisms, belongs to COG1009 (Galperin et al., 2021)

Gene

Coordinates: 205,409 → 206,926

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
• severe growth defect [pubmed|31420537]
• inactivation of [gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF] facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]

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The protein

Catalyzed reaction/ biological activity

• uptake of bicarbonate (with [protein|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|MpsB]) [pubmed|34287032]

Protein family

• complex I subunit 5 family (single member, according to UniProt)

[wiki|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|4HE8] (from Thermus thermophilus, corresponds to aa 70 ... 352, 28.% identity) [pubmed|23417064]
• [AF|P39755]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]-[gene|E04C67FB0EA3FFEC8FB68440F4661505C3DFDA29|ybcC]-[gene|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|ybcF]-[gene|EBE0360D89E50EEBB84CC853C6E4B4053DBF8925|ybcH]-[gene|6E063903E9E3B770CAACA9DF98DFA540FAE6FD3D|ybcI]

  description: [Pubmed|22383849]

additional information

• highly expressed at high iron concentrations [pubmed|31420537]

Biological materials

Mutant

• BKE01830 (Δ[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT,  downstream forward: _UP4_ATTTCATAAGGAGCTAATCT
• BKK01830 (Δ[gene|CCDDC414E266D86B9027EA67EA565D87357023B3|ndhF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT,  downstream forward: _UP4_ATTTCATAAGGAGCTAATCT

References

• 18763711,31420537,23417064,33481774,25448817,31399577,34287032,34730408