amiE

168

N-acetylmuramyl-L-alanine amidase

locus: BSU_01670

Molecular weight: 49.14 kDa

pI: 9.35

Protein length: 441 aa

Gene length: 1326 bp

function: recycling of cell wall material

product: N-acetylmuramyl-L-alanine amidase

essential: no

synonyms: amiE, ybbE

List of homologs in different organisms, belongs to COG1680 (Galperin et al., 2021)

Gene

Coordinates: 188,408 → 189,733

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The protein

Catalyzed reaction/ biological activity

• hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide [Pubmed|20400549]
• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides

Protein family

• peptidase S12 family (single member, according to UniProt)

Structure

• [PDB|4IVK] (from uncultured bacterium, 25% identity) [pubmed|23737193]
• [AF|O05213]

Expression and Regulation

Operons

• genes: [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|namZ]

  description: [Pubmed|22383849]

  regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]

  regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]: repression, [pubmed|30038046], in [regulon|protein:125872506C400CB6B3DAEA8B5130B064DFBA4494|murR regulon]

Biological materials

Mutant

• BKE01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG,  downstream forward: _UP4_TGAATGATTAGACGATAGGA
• BKK01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG,  downstream forward: _UP4_TGAATGATTAGACGATAGGA

References

• 10627040,20400549,22383849,23737193,30038046