shfA

168

sporulation heterogeneity factor Antidote, required for sporulation at a late stage

locus: BSU_00610

Molecular weight: 24.95 kDa

pI: 10.74

Protein length: 211 aa

Gene length: 636 bp

function: control of sporulation entry in neighbor cells

product: sporulation heterogeneity factor Antidote

essential: no

synonyms: yabQ

List of homologs in different organisms, belongs to COG5869 (Galperin et al., 2021)

Gene

Coordinates: 68,515  69,150

Phenotypes of a mutant

• inactivation of [gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA] reduces sporulation efficiency to 0.1% that of wild type cells [Pubmed|26735940], this can be suppressed by inactivation of [gene|BA2FA842DAB19E32F48380F56BB772D7E60F2D28|shfP] [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv]

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The protein

Structure

• [AF|P37559]

[wiki|Localization]

• forespore outer membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|D6B1837075ECBEE1FEAC704DDB6795336092897F|yabM]-[gene|52D48F22CB53421EE30970D34B4861E2F8A5893D|yabN]-[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP]-[gene|035B5486A3887FC71ACC0A8FB43ED77DC44E12EA|yabP]-[gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]-[gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC]-[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]

  description: [Pubmed|11283287]

  regulation

  • expressed during sporulation ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|11283287]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,11283287], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|12207695], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|12207695], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]

• genes: [gene|035B5486A3887FC71ACC0A8FB43ED77DC44E12EA|yabP]-[gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]-[gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC]-[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]

  description: [Pubmed|11283287]

  regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|11283287]

  additional information

  • there are about 50,000 molecules of DivIC per cell [http://www.ncbi.nlm.nih.gov/sites/entrez/9426141 PubMed]

Biological materials

Mutant

• MGNA-B918 (yabQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1917 NBRP B. subtilis, Japan]
• BKE00610 ([gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTATAGAATTGTGTCGTCA,  downstream forward: _UP4_AGATGAAAGGAGGACCGTCT
• BKK00610 ([gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTATAGAATTGTGTCGTCA,  downstream forward: _UP4_AGATGAAAGGAGGACCGTCT

References

• 11283287,10869437,15231775,26735940,35638784