Difference between revisions of "AsnO"

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=The gene=
 
=The gene=

Revision as of 15:53, 22 June 2009

  • Description: asparagine synthase (glutamine-hydrolysing)

Gene name asnO
Synonyms yisO, yucB
Essential no
Product asparagine synthase (glutamine-hydrolysing))
Function biosynthesis of asparagine
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 70 kDa, 5.696
Gene length, protein length 1842 bp, 614 aa
Immediate neighbours yisN, yizA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AsnO context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU10790

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)
  • Protein family: glutamine amidotransferase type-2 domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: expressed during sporulation (SigE)
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)