asnH

168

asparagine synthetase (glutamine-hydrolysing)

locus: BSU_39920

Molecular weight: 85.66 kDa

pI: 5.53

Protein length: 747 aa

Gene length: 2244 bp

function: biosynthesis of asparagine

product: asparagine synthetase (glutamine-hydrolysing)

essential: no

ec: 6.3.5.4

synonyms: asnH, yxaN

List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)

Gene

Coordinates: 4,098,926  4,101,169

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The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-aspartate + L-glutamine --> AMP + diphosphate + H⁺ + L-asparagine + L-glutamate (according to UniProt)

Protein family

• asparagine synthetase family (with [protein|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB] and [protein|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO], according to UniProt)

[wiki|Domains]

• [wiki|Glutamine amidotransferase type-2 domain] (aa 2-218) (according to UniProt)

Structure

• [PDB|1CT9] (from E. coli, corresponds to aa 30 ... 537, 27% identity) [pubmed|10587437]
• [AF|P42113]

Biological materials

Mutant

• GP4146 (''asnH''::''ermC''), available in  [wiki|Jörg Stülke]'s lab
• MGNA-B689 (asnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1688 NBRP B. subtilis, Japan]
• BKE39920 ([gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTCTCCCTCCCAATA,  downstream forward: _UP4_AATCTGCCAGAAGGAGCATA
• BKK39920 ([gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTCTCCCTCCCAATA,  downstream forward: _UP4_AATCTGCCAGAAGGAGCATA

References

Reviews

• 12859215,11395405

Original Publications

• 20185509,10746760,12618455,10498721,12618455,15101989,10587437