AsnB

• Description: asparagine synthase (glutamine-hydrolysing)
  
  

Gene name	asnB
Synonyms	asn
Essential	no
Product	asparagine synthase (glutamine-hydrolysing))
Function	biosynthesis of asparagine
Gene expression levels in SubtiExpress: asnB
Metabolic function and regulation of this protein in SubtiPathways: asnB
MW, pI	72 kDa, 5.615
Gene length, protein length	1896 bp, 632 aa
Immediate neighbours	ytnA, metK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU30540

Database entries

• BsubCyc: BSU30540

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H₂O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)

• Protein family: asparagine synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30540

• Structure:

• UniProt: P54420

• KEGG entry: [3]

• E.C. number: 6.3.5.4

Expression and regulation

• Operon: asnB-ytnA PubMed

• Expression browser: asnB PubMed

• Sigma factor:

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 289 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3794 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)