AroK

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  • Description: shikimate kinase

Gene name aroK
Synonyms aroI
Essential no
Product shikimate kinase
Function biosynthesis of aromatic amino acids
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 21 kDa, 5.969
Gene length, protein length 558 bp, 186 aa
Immediate neighbours tmrB, ycgJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AroK context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU03150

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + shikimate = ADP + shikimate 3-phosphate (according to Swiss-Prot)
  • Protein family: shikimate kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: aroK (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

L Huang, A L Montoya, E W Nester
Purification and characterization of shikimate kinase enzyme activity in Bacillus subtilis.
J Biol Chem: 1975, 250(19);7675-81
[PubMed:170268] [WorldCat.org] (P p)

J A Hoch, E W Nester
Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.
J Bacteriol: 1973, 116(1);59-66
[PubMed:4200844] [WorldCat.org] [DOI] (P p)

E W Nester, R A Jensen, D S Nasser
Regulation of enzyme synthesis in the aromatic amino acid pathway of Bacillus subtilus.
J Bacteriol: 1969, 97(1);83-90
[PubMed:4974400] [WorldCat.org] [DOI] (P p)