AroK

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  • Description: shikimate kinase

Gene name aroK
Synonyms aroI
Essential no
Product shikimate kinase
Function biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroK
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 21 kDa, 5.969
Gene length, protein length 558 bp, 186 aa
Immediate neighbours tmrB, ycgJ
Sequences Protein DNA DNA_with_flanks
Genetic context
AroK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AroK expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU03150

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + shikimate = ADP + shikimate 3-phosphate (according to Swiss-Prot)
  • Protein family: shikimate kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: aroK (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Cuauhtemoc Licona-Cassani, Alvaro R Lara, Natividad Cabrera-Valladares, Adelfo Escalante, Georgina Hernández-Chávez, Alfredo Martinez, Francisco Bolívar, Guillermo Gosset
Inactivation of pyruvate kinase or the phosphoenolpyruvate: sugar phosphotransferase system increases shikimic and dehydroshikimic acid yields from glucose in Bacillus subtilis.
J Mol Microbiol Biotechnol: 2014, 24(1);37-45
[PubMed:24158146] [WorldCat.org] [DOI] (I p)

L Huang, A L Montoya, E W Nester
Purification and characterization of shikimate kinase enzyme activity in Bacillus subtilis.
J Biol Chem: 1975, 250(19);7675-81
[PubMed:170268] [WorldCat.org] (P p)

J A Hoch, E W Nester
Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.
J Bacteriol: 1973, 116(1);59-66
[PubMed:4200844] [WorldCat.org] [DOI] (P p)

E W Nester, R A Jensen, D S Nasser
Regulation of enzyme synthesis in the aromatic amino acid pathway of Bacillus subtilus.
J Bacteriol: 1969, 97(1);83-90
[PubMed:4974400] [WorldCat.org] [DOI] (P p)