AroK

• Description: shikimate kinase
  
  

Gene name	aroK
Synonyms	aroI
Essential	no
Product	shikimate kinase
Function	biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroK
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	21 kDa, 5.969
Gene length, protein length	558 bp, 186 aa
Immediate neighbours	tmrB, ycgJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU03150

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + shikimate = ADP + shikimate 3-phosphate (according to Swiss-Prot)

• Protein family: shikimate kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37944

• KEGG entry: [3]

• E.C. number: 2.7.1.71

Expression and regulation

• Operon: aroK (according to DBTBS)

• Expression browser: aroK PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Cuauhtemoc Licona-Cassani, Alvaro R Lara, Natividad Cabrera-Valladares, Adelfo Escalante, Georgina Hernández-Chávez, Alfredo Martinez, Francisco Bolívar, Guillermo Gosset
Inactivation of pyruvate kinase or the phosphoenolpyruvate: sugar phosphotransferase system increases shikimic and dehydroshikimic acid yields from glucose in Bacillus subtilis.
J Mol Microbiol Biotechnol: 2014, 24(1);37-45
[PubMed:24158146] [WorldCat.org] [DOI] (I p)

L Huang, A L Montoya, E W Nester
Purification and characterization of shikimate kinase enzyme activity in Bacillus subtilis.
J Biol Chem: 1975, 250(19);7675-81
[PubMed:170268] [WorldCat.org] (P p)

J A Hoch, E W Nester
Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.
J Bacteriol: 1973, 116(1);59-66
[PubMed:4200844] [WorldCat.org] [DOI] (P p)

E W Nester, R A Jensen, D S Nasser
Regulation of enzyme synthesis in the aromatic amino acid pathway of Bacillus subtilus.
J Bacteriol: 1969, 97(1);83-90
[PubMed:4974400] [WorldCat.org] [DOI] (P p)