AroF

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  • Description: chorismate synthase

Gene name aroF
Synonyms
Essential no
Product chorismate synthase
Function biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroF
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 40 kDa, 6.578
Gene length, protein length 1107 bp, 369 aa
Immediate neighbours aroB, cheR
Sequences Protein DNA DNA_with_flanks
Genetic context
AroF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AroF expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22710

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate (according to Swiss-Prot)
  • Protein family: acetokinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-127 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2O11 (from Mycobacterium tuberculosis h37rv mutant, 42% identity, 58% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

S O Hoch
Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis.
J Bacteriol: 1974, 117(1);315-7
[PubMed:4202998] [WorldCat.org] [DOI] (P p)