aroF

168

chorismate synthase

locus: BSU_22710

Molecular weight: 40.04 kDa

pI: 6.58

Protein length: 390 aa

Gene length: 1173 bp

function: biosynthesis of aromatic amino acids

product: chorismate synthase

essential: yes

ec: 4.2.3.5

synonyms: aroF

List of homologs in different organisms, belongs to COG0082 (Galperin et al., 2021)

Gene

Coordinates: 2,379,100  2,380,272

Phenotypes of a mutant

• essential [Pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• 5-O-(1-carboxyvinyl)-3-phosphoshikimate --> chorismate + phosphate (according to UniProt)

Protein family

• chorismate synthase family (single member, according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|2O11] (from ''Mycobacterium tuberculosis h37rv mutant'', 42% identity, 58% similarity)
• [AF|P31104]

Modification

• phosphorylated on Arg-127 [Pubmed|22517742]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE22710 ([gene|9712D0E655289C97315314BDEE1A0DF2ED2ADCA2|aroF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGACTTCTTCTCCCTTCA,  downstream forward: _UP4_AGAAAACTGTCGAGGGAATT

References

Reviews

• 12966138

Original Publications

• 4202998,22517742,28189581,21815947