aroE

aroE
168

5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, 3-phosphoshikimate 1-carboxyvinyltransferase

locus
BSU_22600
Molecular weight
45.08 kDa
pI
6.34
Protein length
Gene length
function
biosynthesis of aromatic amino acids
product
5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase
essential
yes
ec
2.5.1.19
synonyms
aroE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0128 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,367,954 → 2,369,240
Phenotypes of a mutant
essential on LB medium [Pubmed|28189581,30666812], but not on BHI medium [pubmed|37822042]
the mutant is able to grow on LB if menachinone is added to the medium [pubmed|37822042]
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The protein
Catalyzed reaction/ biological activity
3-phosphoshikimate + phosphoenolpyruvate --> 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate (according to UniProt)
Protein family
EPSP synthase family (with [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] and [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB], according to UniProt)
Structure
[PDB|3RMT] (from ''B. halodurans'', 68% identity, 87% similarity)
[AF|P20691]
Effectors of protein activity
inhibited by glyphosate [pubmed|30666812]
Expression and Regulation
Operons
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]-[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
Open in new tab

[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]→[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]

2024-03-15 18:03:38

Jstuelk

64

a3b37416002f0f1e65847bd8d15ba18b53400752

AA88BDB7192FA5467301E36C102EEEBB21BEDA86

genes
[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
description
[Pubmed|21815947]
additional information
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Open in new tab

[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]→[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]

2024-03-10 09:00:45

Jstuelk

77

b502e73a1da6c7ae180abdcda54d9a19e58e7035

F5ED1AEC5B7628117F3A30DD1642886538E7A56A

Biological materials
Mutant
BKE22600 (Δ[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22600 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_CTTATCTCGTTTCATTTTTT,  downstream forward: _UP4_TGAAGTTTTACTTCAGGATT
Expression vectors
for expression in ''B. subtilis'', in [wiki|pBQ200]: piGEM2,  available in [wiki|Fabian Commichau]'s lab
labs
[wiki|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]
References
Reviews
12966138
Original Publications
3924737,3106153,6436812,1551827,8419914,21815947,28189581,30666812,3111378,7744055,37822042

CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1

Page visits: 3699

Time of last update: 2024-03-28 10:47:34

Author of last update: Jstuelk