aroE

168

5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, 3-phosphoshikimate 1-carboxyvinyltransferase

locus: BSU_22600

Molecular weight: 45.08 kDa

pI: 6.34

Protein length: 428 aa

Gene length: 1287 bp

function: biosynthesis of aromatic amino acids

product: 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase

essential: yes

ec: 2.5.1.19

synonyms: aroE

List of homologs in different organisms, belongs to COG0128 (Galperin et al., 2021)

Gene

Coordinates: 2,367,954 → 2,369,240

Phenotypes of a mutant

• essential on LB medium [Pubmed|28189581,30666812], but not on BHI medium [pubmed|37822042]
• the mutant is able to grow on LB if menachinone is added to the medium [pubmed|37822042]

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The protein

Catalyzed reaction/ biological activity

• 3-phosphoshikimate + phosphoenolpyruvate --> 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate (according to UniProt)

Protein family

• EPSP synthase family (with [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] and [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB], according to UniProt)

Structure

• [PDB|3RMT] (from ''B. halodurans'', 68% identity, 87% similarity)
• [AF|P20691]

Effectors of protein activity

• inhibited by glyphosate [pubmed|30666812]

Biological materials

Mutant

• BKE22600 (Δ[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22600 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_CTTATCTCGTTTCATTTTTT,  downstream forward: _UP4_TGAAGTTTTACTTCAGGATT

Expression vectors

• for expression in ''B. subtilis'', in [wiki|pBQ200]: piGEM2,  available in [wiki|Fabian Commichau]'s lab

labs

• [wiki|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

References

Reviews

• 12966138

Original Publications

• 3924737,3106153,6436812,1551827,8419914,21815947,28189581,30666812,3111378,7744055,37822042