AroD

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  • Description: shikimate dehydrogenase

Gene name aroD
Synonyms
Essential no
Product shikimate dehydrogenase
Function biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroD
Metabolic function and regulation of this protein in SubtiPathways:
aroD
MW, pI 30 kDa, 6.077
Gene length, protein length 840 bp, 280 aa
Immediate neighbours yqeI, yqeH
Sequences Protein DNA DNA_with_flanks
Genetic context
AroD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AroD expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25660

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Shikimate + NADP+ = 3-dehydroshikimate + NADPH (according to Swiss-Prot)
  • Protein family: shikimate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2HK8 (from Aquifex aeolicus, 42% identity, 61% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Dong-Feng Liu, Guo-Min Ai, Qing-Xiang Zheng, Chang Liu, Cheng-Ying Jiang, Li-Xia Liu, Bo Zhang, Yi-Ming Liu, Chen Yang, Shuang-Jiang Liu
Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains.
Microb Cell Fact: 2014, 13(1);40
[PubMed:24628944] [WorldCat.org] [DOI] (I e)

J Bergsma, K E Meihuizen, W Van Oeveren, W N Konings
Restoration of NADH oxidation with menaquinones and menaquinone analogues in membrane vesicles from the menaquinone-deficient Bacillus subtilis aroD.
Eur J Biochem: 1982, 125(3);651-7
[PubMed:6811271] [WorldCat.org] [DOI] (P p)