aroD

aroD
168

shikimate dehydrogenase

locus
BSU_25660
Molecular weight
30.49 kDa
pI
6.08
Protein length
Gene length
function
biosynthesis of aromatic amino acids
product
shikimate dehydrogenase
essential
no
ec
1.1.1.25
synonyms
aroD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0169 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,644,630  2,645,472
Phenotypes of a mutant
slow growth [pubmed|31358606]
50% reduction in mo5U tRNA levels (because prephenate, the product formed by [protein|5C2F7F9F5FCBFEDF77AC41725EFDA0FAF0FC3BC1|aroH], is used as hydroxyl donor for partial ho5U synthesis) [pubmed|31358606]
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The protein
Catalyzed reaction/ biological activity
NADP+ + shikimate --> 3-dehydroshikimate + H+ + NADPH (according UniProt)
Protein family
shikimate dehydrogenase family (single member, according to UniProt)
Structure
[PDB|2HK8] (from ''Aquifex aeolicus'', 42% identity, 61% similarity) [Pubmed|17649975]
[AF|P54374]
Expression and Regulation
Operons
genes
[gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
description
[Pubmed|22383849]
expression
expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
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[gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]→[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]

2024-03-10 08:59:20

Jstuelk

76

16D917C7583868297F7E4E92450A5FA00BB1FF6D

20160DA9FBD3CC875D87545DE18F83BF8B1D0550

Biological materials
Mutant
BKE25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25660 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT,  downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
BKK25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT,  downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
References
6811271,24628944,22383849,31358606

776D79C45DADA631D4A75B0773CFF471A87D9EEC

Page visits: 2786

Time of last update: 2024-03-29 12:00:36

Author of last update: Melvin.boenninger