aroD
168
shikimate dehydrogenase
locus
BSU_25660
Molecular weight
30.49 kDa
pI
6.08
function
biosynthesis of aromatic amino acids
product
shikimate dehydrogenase
essential
no
ec
1.1.1.25
synonyms
aroD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0169 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,644,630 2,645,472
Phenotypes of a mutant
slow growth [pubmed|31358606]
50% reduction in mo5U tRNA levels (because prephenate, the product formed by [protein|5C2F7F9F5FCBFEDF77AC41725EFDA0FAF0FC3BC1|aroH], is used as hydroxyl donor for partial ho5U synthesis) [pubmed|31358606]
The protein
Catalyzed reaction/ biological activity
NADP+ + shikimate --> 3-dehydroshikimate + H+ + NADPH (according UniProt)
Protein family
shikimate dehydrogenase family (single member, according to UniProt)
Structure
[PDB|2HK8] (from ''Aquifex aeolicus'', 42% identity, 61% similarity) [Pubmed|17649975]
[AF|P54374]
Biological materials
Mutant
BKE25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25660 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
BKK25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
References
Page visits: 2911
Time of last update: 2024-04-24 02:44:05
Author of last update: Melvin.boenninger