Difference between revisions of "AroD"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''
 
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=aroD_2644630_2645472_-1 aroD] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=aroD_2644630_2645472_-1 aroD] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''Regulation:'''  
 
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=References=
 
=References=
  
<pubmed>6811271,, </pubmed>
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<pubmed>6811271, 24628944, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:54, 19 March 2014

  • Description: shikimate dehydrogenase

Gene name aroD
Synonyms
Essential no
Product shikimate dehydrogenase
Function biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroD
Metabolic function and regulation of this protein in SubtiPathways:
aroD
MW, pI 30 kDa, 6.077
Gene length, protein length 840 bp, 280 aa
Immediate neighbours yqeI, yqeH
Sequences Protein DNA DNA_with_flanks
Genetic context
AroD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AroD expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25660

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Shikimate + NADP+ = 3-dehydroshikimate + NADPH (according to Swiss-Prot)
  • Protein family: shikimate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2HK8 (from Aquifex aeolicus, 42% identity, 61% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Dong-Feng Liu, Guo-Min Ai, Qing-Xiang Zheng, Chang Liu, Cheng-Ying Jiang, Li-Xia Liu, Bo Zhang, Yi-Ming Liu, Chen Yang, Shuang-Jiang Liu
Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains.
Microb Cell Fact: 2014, 13(1);40
[PubMed:24628944] [WorldCat.org] [DOI] (I e)

J Bergsma, K E Meihuizen, W Van Oeveren, W N Konings
Restoration of NADH oxidation with menaquinones and menaquinone analogues in membrane vesicles from the menaquinone-deficient Bacillus subtilis aroD.
Eur J Biochem: 1982, 125(3);651-7
[PubMed:6811271] [WorldCat.org] [DOI] (P p)