ArgD

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  • Description: acetylornithine transaminase

Gene name argD
Synonyms
Essential no
Product acetylornithine transaminase
Function biosynthesis of arginine
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 40 kDa, 5.929
Gene length, protein length 1155 bp, 385 aa
Immediate neighbours argB, carA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ArgD context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU11220

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate (according to Swiss-Prot)
  • Protein family: ArgD subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed, repressed by arginine (AhrC)
  • Regulatory mechanism: AhrC: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)